Epigenética Aplicada

Ementa:

A disciplina visa introduzir os fundamentos teóricos e aplicação das principais técnicas de biologia molecular e ensaios bioquímicos aplicados ao estudo dos mecanismos Epigenéticos, bem como possibilitar os alunos para escolha da(s) técnica(s) mais adequadas para delineamento de projetos relacionados ao estudo dos mecanismos Epigenéticos.

Bibliografia:

Buenrostro, J.D., Wu, B., Chang, H.Y., Greenleaf, W.J., 2015. ATAC-seq: A Method for Assaying Chromatin Accessibility Genome-Wide. Curr. Protoc. Mol. Biol. 109, 21.29.1-21.29.9. https://doi.org/10.1002/0471142727.mb2129s109

Garrido-Cardenas JA, Garcia-Maroto F, Alvarez-Bermejo JA, Manzano-Agugliaro F. DNA Sequencing Sensors: An Overview. Sensors (Basel). 2017 Mar 14;17(3):588. doi: 10.3390/s17030588. PMID: 28335417; PMCID: PMC5375874.

Nestor, C.E., Ottaviano, R., Reddington, J., Sproul, D., Reinhardt, D., Dunican, D., Katz, E., Dixon, J.M., Harrison, D.J., Meehan, R.R., 2012. Tissue type is a major modifier of the 5-hydroxymethylcytosine content of human genes. Genome Res. 22, 467-477. https://doi.org/10.1101/gr.126417.111

Picelli, S., 2017. Single-cell RNA-sequencing: The future of genome biology is now. RNA Biol. 14, 637-650. https://doi.org/10.1080/15476286.2016.1201618

Pidsley, R., Zotenko, E., Peters, T.J., Lawrence, M.G., Risbridger, G.P., Molloy, P., Van Djik, S., Muhlhausler, B., Stirzaker, C., Clark, S.J., 2016. Critical evaluation of the Illumina MethylationEPIC BeadChip microarray for whole-genome DNA methylation profiling. Genome Biol. 17, 208. https://doi.org/10.1186/s13059-016-1066-1

Ramalho-Carvalho, J., Henrique, R., Jerónimo, C., 2018. Methylation-Specific PCR. Methods Mol. Biol. 1708, 447-472. https://doi.org/10.1007/978-1-4939-7481-8_23

Varinli, H., Statham, A.L., Clark, S.J., Molloy, P.L., Ross, J.P., 2015. COBRA-Seq: Sensitive and Quantitative Methylome Profiling. Genes (Basel). 6, 1140-1163. https://doi.org/10.3390/genes6041140

Wang, K.C., Chang, H.Y., 2018. Epigenomics: Technologies and Applications. Circ. Res. 122, 1191-1199. https://doi.org/10.1161/CIRCRESAHA.118.310998.