Ementa:
Testes diagnósticos baseados na utilização da Biologia Molecular. Emprego e objetivos das diferentes técnicas de Biologia Molecular utilizadas em Odontologia. Aplicação científica de técnicas como PCR Real Time, DNA-DNA Checkerboard e Clonagem de DNA. Perspectivas do uso de Biologia Molecular para o desenvolvimento da prática odontológica.
Bibliografia:
ALBERTS B et al. Biologia Molecular da Célula. Tradução Ana Letícia de Souza Vanz et al. 6ª ed. Porto Alegre: Artmed; 2017.
ZAHA A, FERREIRA HB, PASSAGLIA L M. P. Biologia Molecular Básica. 5ª ed. Porto Alegre: Artmed; 2014.
Kc S, Wang Xz, Gallagher Je. Diagnostic sensitivity and specificity of host-derived salivary biomarkers in periodontal disease amongst adults: Systematic review. J Clin Periodontol. 2020 Mar;47(3):289-308.
Arias-Bujanda N, Regueira-Iglesias A, Balsa-Castro C, Nibali L, Donos N, Tomás I. Accuracy of single molecular biomarkers in gingival crevicular fluid for the diagnosis of periodontitis: A systematic review and meta-analysis. J Clin Periodontol. 2019 Dec;46(12):1166-1182.
Gupta S, Chhina S, Arora SA. A systematic review of biomarkers of gingival crevicular fluid: Their predictive role in diagnosis of periodontal disease status. J Oral Biol Craniofac Res. 2018 May-Aug;8(2):98-104
Dabdoub SM, Tsigarida AA, Kumar PS. Patient-specific Analysis of Periodontal and Peri-implant Microbiomes. J Dent Res. 2013 Dec;92(12 Suppl):168S-75S. doi: 10.1177/0022034513504950.
Violant D, Galofré M, Nart J, Teles RP. In vitro evaluation of a multispecies oral biofilm on different implant surfaces. Biomed Mater. 2014 Jun;9(3):035007.
Chaves de Souza JA, Frasnelli SC, Curylofo-Zotti FA, Ávila-Campos MJ, Spolidório LC, Zamboni DS, Graves DT, Rossa C Jr. NOD1 in the modulation of host-microbe interactions and inflammatory bone resorption in the periodontal disease model. Immunology. 2016;149(4):374-385.
Grdović N, Rajić J, Petrović SM, Dinić S, Uskoković A, Mihailović M, Jovanović JA, Tolić A, Pucar A, Milašin J, Vidaković M. Association of CXCL12 gene promoter methylation with periodontitis in patients with diabetes mellitus type 2. Arch Oral Biol. 2016;72:124-133.
Periódicos:
Journal of Periodontology
Journal of Periodontal Research
Journal of Clinical Periodontology
Periodontology 2000
International Journal of Periodontics and Restorative Dentistry